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Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species
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SYSNO ASEP 0315061 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species Author(s) Nováková, D. (CZ)
Hubálek, Zdeněk (UBO-W) RID, SAI, ORCID
Sedláček, I. (CZ)Source Title XII. International Congress of Bacteriology and Applied Microbiology, 5-9 August 2008, Istanbul: Abstract Book. - Istanbul : International Union of Microbiological Societies, 2008
S. 151Number of pages 1 s. Action International Congress of Bacteriology and Applied Microbiology /12./ Event date 05.08.2008-09.08.2008 VEvent location Istanbul Country TR - Turkey Event type WRD Language eng - English Country TR - Turkey Keywords Staphylococcus ; common vole Subject RIV EE - Microbiology, Virology CEZ AV0Z60930519 - UBO-W (2005-2011) Annotation Two strains of Gram-positive cocci isolated from viscera of common voles (Microtus arvalis) were studied by phenotyping and genotyping. Biochemical features were tested by Api Staph kits (bioMérieux) and by conventional tests. Detailed characterization was done by ribotyping, whole-cell protein analysis and rep-PCR using (GTG)5 primer. The phylogenetic position was analysed by partial 16S rRNA sequencing. Both catalase positive, oxidase negative strains, resistant to bacitracin and sensitive to novobiocin and furazolidon were identified as Staphylococcus sp. Strains did not show unambiguous species identification based on phenotyping. Analysed strains revealed high mutual similarity of ribotype (EcoRI 88.9% and HindIII 78.6%), protein (95.6%) as well as rep-PCR (98.2%) fingerprints and concurrently were clearly distinct from all validly described staphylococcal taxa. The isolates showed the same partial 16S rRNA sequences, and the maximum sequence similarities were obtained with Staphylococcus muscae (98.3%) and Staphylococcus chromogenes (98.2%) type cultures - their sequences differed in 7 and 11 nucleotides, respectively. The obtained results proved that the tested isolates are two different strains representing probably a novel staphylococcal species. The study was partly supported by projects MSM0021622416 and AV0Z60960519. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2009
Number of the records: 1