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Gama Tubulin and its role in Arabidopsis development
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SYSNO ASEP 0305115 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Gama Tubulin and its role in Arabidopsis development Title Gama Tubulin a jeho role u Arabidopsis Author(s) Cenklová, Věra (UEB-Q)
Doskočilová, Anna (MBU-M)
Petrovská (UEB-Q)
Gallová, Barbora (UEB-Q)
Kofroňová, Olga (MBU-M) RID, ORCID
Benada, Oldřich (MBU-M) ORCID, RID
Binarová, Pavla (MBU-M) RID, ORCIDSource Title -. -, 2007
S. 79Number of pages 1 s. Action International Symposium on Plant Neurobiology/3./ Event date 14.05.2007-18.05.2007 VEvent location Štrbské Pleso Country SK - Slovakia Event type WRD Language eng - English Country CZ - Czech Republic Keywords gama tubulin ; Arabidopsis development Subject RIV EB - Genetics ; Molecular Biology Annotation We found that inducible RNAi depletion of γ-tubulin led to serious distortions of development in A. thaliana seedlings. Cells with decreased levels of γ-tubulin could progress through mitosis, but late mitotic events and cytokinesis were strongly affected. Particularly, we observed that polar distribution of γ tubulin during late mitosis was disturbed and the phragmoplast formation failed. In contrast to the control cells, long anaphase spindles persisted between separated nuclei in RNAi cells. The cell plate formation sites were often misaligned. These discrepancies in late mitosis and cytokinesis often resulted in bi or multi-nuclear cells and disruption of regular cell files and some morphogenic changes were observed. Strict developmental pattern of stomata was disrupted and clusters of two to four stomata were observed in RNAi expressing plants with reduced γ-tubulin levels. In addition the cytokinetic defects of guard cells were found when γ tubulin was severely depleted. Dorsoventral polarity during leaf development was disturbed in seedlings with reduced γ tubulin levels. Ectopic root hairs formation was observed in cells with randomized microtubules, anisotropic growth of root hairs was disturbed, formation of two growth axes was often observed. We suggest that some functions of γ tubulin that are important for cytokinesis, cell specification and polar growth might be microtubule independent and require further analysis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2008
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