Number of the records: 1
Measurement of Intensity Profile in the Focal Region of Microscope Objective
- 1.0205424 - UPT-D 20010064 RIV CZ eng C - Conference Paper (international conference)
Šerý, Mojmír - Ježek, Jan - Jákl, Petr - Jonáš, Alexandr - Zemánek, Pavel - Liška, M.
Measurement of Intensity Profile in the Focal Region of Microscope Objective.
Sborník příspěvků konference Nové trendy ve fyzice. Vol. 2. Brno: Vysoké učení technické v Brně, 2001 - (Štrunc, M.; Hradilová, E.), s. 410-414. ISBN 80-214-1992-X.
[Nové trendy ve fyzice. Brno (CZ), 15.11.2001-16.11.2001]
Institutional research plan: CEZ:AV0Z2065902
Keywords : two-photon fluorescence * focused beam * intensity measurement
Subject RIV: BH - Optics, Masers, Lasers
This work describes an experimental method for the measurement of the profile of the laser beam focused by an immersion microscope objective. The method makes use of the two-photon excited fluorescence emitted by a 200 nm dyed polystyrene bead fixed to the cover slip. A piezo driven stage is used for scanning of the bead through the focal volume of an infrared laser beam and the excited two-photon fluorescence is detected by a photomultiplier.
Permanent Link: http://hdl.handle.net/11104/0101037
Number of the records: 1