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Multivalent weak electrolytes - risky background electrolytes for capillary zone electrophoresis
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SYSNO ASEP 0162399 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Multivalent weak electrolytes - risky background electrolytes for capillary zone electrophoresis Author(s) Beckers, J. L. (NL)
Boček, Petr (UIACH-O) RIDSource Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 23, č. 12 (2002), s. 1942-1946Number of pages 5 s. Language eng - English Country DE - Germany Keywords background electrolytes ; capillary zone electrophoresis ; multivalent electrolytes Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA203/99/0044 GA ČR - Czech Science Foundation (CSF) GA203/02/0023 GA ČR - Czech Science Foundation (CSF) GA203/01/0401 GA ČR - Czech Science Foundation (CSF) IAA4031703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA4031103 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation Multivalent weak acids and bases are useful components of buffers in electrophoresis. The use of such buffers as background electrolytes (BGEs) in capillary zone electrophoresis (CZE) is, however, risky due to the existence of unsafe regions in the analytical window of the separation. This contribution discusses the problems and shows that multivalent weak species in BGEs bring about the same effects as mixtures of two independent co-ions, i.e., the presence of two centers of symmetry in the electropherograms and the existence of a migrating system zone with a mobility in between these two centers of symmetry. The system zone deteriorates the analytical separation and detection of the analytes in its neighborhood. Illustrative experimental examples for both cationic and anionic CZE are shown and related discussion is given. Finally, some basic rules are formulated to avoid the preparation of risky BGEs. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2003
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