The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in iPseudomonas aeruginosa/i

0577003 - MBÚ 2024 RIV CH eng J - Journal Article
Juříková, Tereza - Mácha, Hynek - Lupjanová, Vanda - Pluháček, T. - Marešová, Helena - Papoušková, B. - Luptáková, Dominika - Patil, Rutuja Hiraji - Benada, Oldřich - Grulich, Michal - Palyzová, Andrea
The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in iPseudomonas aeruginosa/i.
Microorganisms. Roč. 11, č. 9 (2023), č. článku 2329. E-ISSN 2076-2607
R&D Projects: GA ČR(CZ) GA21-17044S
Institutional support: RVO:61388971
Keywords : Aspergillus fumigatus * biofilm * microbial interaction * metabolomic analysis * planktonic cell * Pseudomonas aeruginosa * QS system
OECD category: Microbiology
Impact factor: 4.5, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2076-2607/11/9/2329

Pseudomonas aeruginosa is recognized as a significant cause of morbidity and mortality among nosocomial pathogens. In respiratory infections, P. aeruginosa acts not only as a single player but also collaborates with the opportunistic fungal pathogen Aspergillus fumigatus. This study introduced a QS molecule portfolio as a potential new biomarker that affects the secretion of virulence factors and biofilm formation. The quantitative levels of QS molecules, including 3-o-C12-HSL, 3-o-C8-HSL, C4-HSL, C6-HSL, HHQ, PQS, and PYO, measured using mass spectrometry in a monoculture, indicated metabolic changes during the transition from planktonic to sessile cells. In the co-cultures with A. fumigatus, the profile of abundant QS molecules was reduced to 3-o-C12-HSL, C4-HSL, PQS, and PYO. A decrease in C4-HSL by 50% to 170.6 +/- 11.8 ng/mL and an increase 3-o-C12-HSL by 30% up to 784.4 +/- 0.6 ng/mL were detected at the stage of the coverage of the hyphae with bacteria. Using scanning electron microscopy, we showed the morphological stages of the P. aeruginosa biofilm, such as cell aggregates, maturated biofilm, and cell dispersion. qPCR quantification of the genome equivalents of both microorganisms suggested that they exhibited an interplay strategy rather than antagonism. This is the first study demonstrating the quantitative growth-dependent appearance of QS molecule secretion in a monoculture of P. aeruginosa and a co-culture with A. fumigatus.
Permanent Link: https://hdl.handle.net/11104/0347166