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Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts
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SYSNO ASEP 0574417 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts Author(s) Hoboth, Peter (UMG-J) ORCID
Sztacho, Martin (UMG-J) ORCID
Quaas, A. (DE)
Akguel, B. (DE)
Hozák, Pavel (UMG-J) RID, ORCIDNumber of authors 5 Article number 1217637 Source Title Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 11, Jul (2023)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords formalin-fixed paraffin-embedded tissue sections ; STED nanoscopy ; cell nucleus ; human papillomavirus (HPV) ; nuclear architecture ; phosphatidylinositol 4,5-bisphosphate ; nuclear speckles ; quantitative image analysis OECD category Cell biology R&D Projects EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TN02000122 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) TN02000020 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC19048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 001034185000001 EID SCOPUS 85165553482 DOI 10.3389/fcell.2023.1217637 Annotation Introduction: Imaging of human clinical formalin-fixed paraffin-embedded (FFPE) tissue sections provides insights into healthy and diseased states and therefore represents a valuable resource for basic research, as well as for diagnostic and clinical purposes. However, conventional light microscopy does not allow to observe the molecular details of tissue and cell architecture due to the diffraction limit of light. Super-resolution microscopy overcomes this limitation and provides access to the nanoscale details of tissue and cell organization.Methods: Here, we used quantitative multicolor stimulated emission depletion (STED) nanoscopy to study the nanoscale distribution of the nuclear phosphatidylinositol 4,5-bisphosphate (nPI(4,5)P2) with respect to the nuclear speckles (NS) marker SON.Results: Increased nPI(4,5)P2 signals were previously linked to human papillomavirus (HPV)-mediated carcinogenesis, while NS-associated PI(4,5)P2 represents the largest pool of nPI(4,5)P2 visualized by staining and microscopy. The implementation of multicolor STED nanoscopy in human clinical FFPE skin and wart sections allowed us to provide here the quantitative evidence for higher levels of NS-associated PI(4,5)P2 in HPV-induced warts compared to control skin.Discussion: These data expand the previous reports of HPV-induced increase of nPI(4,5)P2 levels and reveal for the first time the functional, tissue-specific localization of nPI(4,5)P2 within NS in clinically relevant samples. Moreover, our approach is widely applicable to other human clinical FFPE tissues as an informative addition to the classical histochemistry. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2024 Electronic address https://www.frontiersin.org/articles/10.3389/fcell.2023.1217637/full
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