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Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
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SYSNO ASEP 0574278 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity Author(s) Koch, S. M. (DE)
Freidank-Pohl, C. (DE)
Siontas, O. (DE)
Cortesao, M. (DE)
Mota, A. (DE)
Runzheimer, K. (DE)
Jung, S. (DE)
Rebrošová, K. (CZ)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Moeller, R. (DE)
Meyer, V. (DE)Number of authors 11 Article number 1233740 Source Title Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 14, 20 July (2023)Number of pages 15 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Aspergillus niger ; melanin ; pyomelanin ; radioprotection ; cosmic radiation ; space exploration Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NU21-05-00341 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 001041737100001 EID SCOPUS 85166760553 DOI 10.3389/fmicb.2023.1233740 Annotation Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2024 Electronic address https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233740/full
Number of the records: 1