Metallacarborane Cluster Anions of the Cobalt Bisdicarbollide-Type as Chaotropic Carriers for Transmembrane and Intracellular Delivery of Cationic Peptides

0573413 - ÚACH 2024 RIV US eng J - Journal Article
Chen, Y. - Barba-Bon, A. - Grüner, Bohumír - Winterhalter, M. - Aksoyoglu, M. A. - Pangeni, S. - Ashjari, M. - Brix, K. - Salluce, G. - Folgar-Cameán, Y. - Montenegro, J. - Nau, W. M.
Metallacarborane Cluster Anions of the Cobalt Bisdicarbollide-Type as Chaotropic Carriers for Transmembrane and Intracellular Delivery of Cationic Peptides.
Journal of the American Chemical Society. Roč. 145, č. 24 (2023), s. 13089-13098. ISSN 0002-7863. E-ISSN 1520-5126
R&D Projects: GA ČR(CZ) GA21-14409S
Institutional support: RVO:61388980
Keywords : Assays * Fluorescence * Membranes * Peptides and proteins * Vesicles
OECD category: Inorganic and nuclear chemistry
Impact factor: 15, year: 2022
Method of publishing: Open access

Cobalt bisdicarbollides (COSANs) are inorganic boron-based anions that have been previously reported to permeate by themselves through lipid bilayer membranes, a propensity that is related to their superchaotropic character. We now introduce their use as selective and efficient molecular carriers of otherwise impermeable hydrophilic oligopeptides through both artificial and cellular membranes, without causing membrane lysis or poration at low micromolar carrier concentrations. COSANs transport not only arginine-rich but also lysine-rich peptides, whereas low-molecular-weight analytes such as amino acids as well as neutral and anionic cargos (phalloidin and BSA) are not transported. In addition to the unsubstituted isomers (known as ortho- and meta-COSAN), four derivatives bearing organic substituents or halogen atoms have been evaluated, and all six of them surpass established carriers such as pyrenebutyrate in terms of activity. U-tube experiments and black lipid membrane conductance measurements establish that the transport across model membranes is mediated by a molecular carrier mechanism. Transport experiments in living cells showed that a fluorescent peptide cargo, FITC-Arg8, is delivered into the cytosol.
Permanent Link: https://hdl.handle.net/11104/0343863


Research data: ACS Publications