Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people

0571513 - BC 2024 RIV GB eng J - Journal Article
Lesiczka, P.M. - Hrazdilová, K. - Hönig, Václav - Modrý, David - Žůrek, L.
Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people.
Parasites & Vectors. Roč. 16, č. 1 (2023), č. článku 80. ISSN 1756-3305. E-ISSN 1756-3305
R&D Projects: GA ČR(CZ) GA21-11661S; GA MŠMT(CZ) LX22NPO5103
Institutional support: RVO:60077344
Keywords : Anaplasma phagocytophilum * Ixodes ricinus * Anaplasmosis * Genetic diversity * Infectious diseases
OECD category: Virology
Impact factor: 3.2, year: 2022
Method of publishing: Open access
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-05654-y

Background Although the tick-borne pathogen Anaplasma phagocytophilum is currently described as a single species, studies using genetic markers can distinguish groups of variants associated with different hosts, pathogenicity, zoonotic potential and biotic and geographic niches. The objective of our study was to investigate the genetic diversity of A. phagocytophilum and Ixodes ricinus ticks attached to people.Methods In collaboration with a commercial diagnostic company, a total of 52 DNA samples were obtained from ticks that tested positive for A. phagocytophilum by quantitative PCR. The genetic profile of each sample was determined using the groEL and ankA genes. Identification of the tick species was confirmed by partial sequencing of the COI subunit and a portion of the TROSPA gene.Results All 52 ticks were identified as I. ricinus. Two protocols of nested PCR amplifying 1293- and 407-bp fragments of groEL of A. phagocytophilum yielded amplicons of the expected size for all 52 samples. Among all sequences, we identified 10 unique genetic variants of groEL belonging to ecotype I and ecotype II. The analysis targeting ankA was successful in 46 of 52 ticks. Among all sequences, we identified 21 unique genetic variants phylogenetically belonging to three clusters.Conclusions Our results indicate that ticks attached to people harbor distant genetic variants of A. phagocytophilum, some of which are not recognized as zoonotic. Further studies are needed to determine the risk of human infection by genetic variants other than those designated as zoonotic.
Permanent Link: https://hdl.handle.net/11104/0343714