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Millipede gut-derived microbes as a potential source of cellulolytic enzymes
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SYSNO ASEP 0571439 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Millipede gut-derived microbes as a potential source of cellulolytic enzymes Author(s) Koubová, Anna (BC-A) ORCID, RID
Lorenc, František (BC-A) RID
Horváthová, Terézia (BC-A) RID, ORCID
Chroňáková, Alica (BC-A) RID, ORCID
Šustr, Vladimír (BC-A) RIDArticle number 169 Source Title World Journal of Microbiology and Biotechnology. - : Springer - ISSN 0959-3993
Roč. 39, č. 7 (2023)Number of pages 17 s. Language eng - English Country DE - Germany Keywords actinobacteria ; cellulase ; diplopoda ; fungi ; intestine ; invertebrates Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA17-22572S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 000976075800002 EID SCOPUS 85154601561 DOI 10.1007/s11274-023-03620-5 Annotation Lignocellulose biomass has recently been considered a cost-effective and renewable energy source within circular economy management. Cellulases are important key enzymes for simple, fast, and clean biomass decomposition. The intestinal tract of millipedes is the environment which can provide promising microbial strains with cellulolytic potential. In the present study, we used the tropical millipede Telodeinopus aoutii as an experimental organism. Within a feeding test in which millipedes were fed with oak and maple leaf litter, we focused on isolating culturable cellulolytic microbiota from the millipede gut. Several growth media selecting for actinobacteria, bacteria, and fungi have been used to cultivate microbial strains with cellulolytic activities. Our results showed that oak-fed millipedes provided a higher number of culturable bacteria and a more diversified microbial community than maple-fed ones. The screening for cellulolytic activity using Congo red revealed that about 30% of bacterial and fungal phylotypes isolated from the gut content of T. aoutii, produced active cellulases in vitro. Actinobacteria Streptomyces and Kitasatospora were the most active cellulolytic genera on Congo red test. In contrast, fungi Aspergillus, Penicillium, Cheatomium, Clonostachys, and Trichoderma showed the highest protein-specific cellulase activity quantified by 4-Methylumbelliferyl β-d-cellobioside (4‐MUC). Our findings provide a basis for future research on the enzyme activities of microbes isolated from the digestive tracts of invertebrates and their biocatalytic role in biomass degradation. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://link.springer.com/article/10.1007/s11274-023-03620-5
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