Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics

Čajka, Tomáš; Hricko, Jiří; Rudl Kulhavá, Lucie; Paučová, Michaela; Nováková, Michaela; Kuda, Ondřej

doi:https://dx.doi.org/10.3390/ijms24031987

Number of the records: 1

Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics

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SYSNO ASEP	0569722
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Optimization of Mobile Phase Modifiers for Fast LC-MS-Based Untargeted Metabolomics and Lipidomics
Author(s)	Čajka, Tomáš (FGU-C)_{RID, ORCID, SAI} Hricko, Jiří (FGU-C) Rudl Kulhavá, Lucie (FGU-C)_{ORCID, RID} Paučová, Michaela (FGU-C) Nováková, Michaela (FGU-C) Kuda, Ondřej (FGU-C)_{RID, ORCID, SAI}
Number of authors	6
Article number	1987
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 24, č. 3 (2023)
Number of pages	14 s.
Language	eng - English
Country	CH - Switzerland
Keywords	metabolomics ; lipidomics ; optimization ; liquid chromatography ; mass spectrometry ; mobile phase ; modifiers ; additives ; LC-MS
OECD category	Analytical chemistry
R&D Projects	NU20-01-00186 GA MZd - Ministry of Health (MZ)
	NU22-02-00161 GA MZd - Ministry of Health (MZ)
	LX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTAUSA19124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA20-21114S GA ČR - Czech Science Foundation (CSF)
	GA21-00477S GA ČR - Czech Science Foundation (CSF)
Research Infrastructure	e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000929591700001
EID SCOPUS	85147981382
DOI	10.3390/ijms24031987
Annotation	Liquid chromatography-mass spectrometry (LC-MS) is the method of choice for the untargeted profiling of biological samples. A multiplatform LC-MS-based approach is needed to screen polar metabolites and lipids comprehensively. Different mobile phase modifiers were tested to improve the electrospray ionization process during metabolomic and lipidomic profiling. For polar metabolites, hydrophilic interaction LC using a mobile phase with 10 mM ammonium formate/0.125% formic acid provided the best performance for amino acids, biogenic amines, sugars, nucleotides, acylcarnitines, and sugar phosphate, while reversed-phase LC (RPLC) with 0.1% formic acid outperformed for organic acids. For lipids, RPLC using a mobile phase with 10 mM ammonium formate or 10 mM ammonium formate with 0.1% formic acid permitted the high signal intensity of various lipid classes ionized in ESI(+) and robust retention times. For ESI(-), the mobile phase with 10 mM ammonium acetate with 0.1% acetic acid represented a reasonable compromise regarding the signal intensity of the detected lipids and the stability of retention times compared to 10 mM ammonium acetate alone or 0.02% acetic acid. Collectively, we show that untargeted methods should be evaluated not only on the total number of features but also based on common metabolites detected by a specific platform along with the long-term stability of retention times.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2024
Electronic address	https://www.mdpi.com/1422-0067/24/3/1987

Number of the records: 1