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Plant material selection, collection, preservation, and storage for nuclear DNA content estimation
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SYSNO ASEP 0566766 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant material selection, collection, preservation, and storage for nuclear DNA content estimation Author(s) Čertner, Martin (BU-J) ORCID
Lučanová, Magdalena (BU-J) RID, ORCID
Sliwinska, E. (PL)
Kolář, Filip (BU-J) ORCID, RID
Loureiro, J. (PT)Source Title Cytometry. Part A. - : Wiley - ISSN 1552-4922
Roč. 101, č. 9 (2022), s. 737-748Number of pages 12 s. Language eng - English Country US - United States Keywords flow cytometry ; nuclear DNA content ; nuclear suspensions Subject RIV EF - Botanics OECD category Plant sciences, botany Method of publishing Limited access Institutional support BU-J - RVO:67985939 UT WOS 000672491100001 EID SCOPUS 85109739806 DOI 10.1002/cyto.a.24482 Annotation In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear DNA content estimation using flow cytometry (FCM). While this certainly opens a wide variety of possible applications of FCM, especially when compared to classical karyological techniques restricted to tissues with active cell division, tissue selection and quality may directly affect the precision (and sometimes even reliability) of FCM measurements. It is usually convenient to first consider the goals of the study to either aim for the highest possible accuracy of estimates (e.g., for inferring genome size, detecting homoploid intraspecific genome size variation, aneuploidy, among others), or to decide that histograms of reasonable resolution provide sufficient information (e.g., ploidy level screening within a single model species). Here, a set of best practices guidelines for selecting the optimal plant tissue for FCM analysis, sampling of material, and material preservation and storage are provided. In addition, factors potentially compromising the quality of FCM estimates of nuclear DNA content and data interpretation are discussed. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2023 Electronic address https://doi.org/10.1002/cyto.a.24482
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