The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells

0564363 - ÚMCH 2023 RIV GB eng J - Journal Article
Gunár, Kristýna - Kotrchová, Lenka - Filipová, Marcela - Krunclová, Tereza - Dydowiczová, Aneta - Pola, Robert - Randárová, Eva - Etrych, Tomáš - Janoušková, Olga
The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells.
Nanomedicine. Roč. 17, č. 19 (2022), s. 1307-1322. ISSN 1743-5889. E-ISSN 1748-6963
R&D Projects: GA MŠMT(CZ) LTAUSA18083
Grant - others:AV ČR(CZ) JSPS-22-01
Program: Bilaterální spolupráce
Institutional support: RVO:61389013
Keywords : cytotoxicity * drug delivery * extracellular vesicles
OECD category: Polymer science
Impact factor: 5.5, year: 2022
Method of publishing: Limited access
https://www.futuremedicine.com/doi/10.2217/nnm-2022-0081

Exosomes are extracellular vesicles with the ability to encapsulate bioactive molecules, such as therapeutics. This study identified a new exosome mediated route of doxorubicin and poly(N-(2-hydroxypropyl)methacrylamide) (pHPMA)-bound doxorubicin trafficking in the tumor mass. Exosome loading was achieved via incubation of the therapeutics with an adherent human breast adenocarcinoma cell line and its derived spheroids. Exosomes were characterized using HPLC, nanoparticle tracking analysis (NTA) and western blotting. The therapeutics were successfully loaded into exosomes. Spheroids secreted significantly more exosomes than adherent cells and showed decreased viability after treatment with therapeutic-loaded exosomes, which confirmed successful transmission. To the best of our knowledge, this study provides the first evidence of pHPMA-drug conjugate secretion by extracellular vesicles.
Permanent Link: https://hdl.handle.net/11104/0336549