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Performance of bat-derived macrophages at different temperatures
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SYSNO ASEP 0561727 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Performance of bat-derived macrophages at different temperatures Author(s) Němcová, M. (CZ)
Seidlová, Veronika (UBO-W)
Zukal, Jan (UBO-W) RID, ORCID, SAI
Dundarova, H. (BG)
Zukalová, K. (CZ)
Pikula, J. (CZ)Number of authors 6 Article number 978756 Source Title Frontiers in Veterinary Science. - : Frontiers Media
Roč. 9, SEP (2022)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords Chiroptera (bats) ; in vitro model ; hibernation ; phagocytic activity ; temperature-dependent proliferation ; daily torpor ; macrophage biology Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine OECD category Zoology Method of publishing Open access Institutional support UBO-W - RVO:68081766 UT WOS 000858548200001 EID SCOPUS 85138833530 DOI 10.3389/fvets.2022.978756 Annotation Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37 degrees C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to20 degrees C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37 degrees C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8 degrees C and epithelial-like, spindle-shaped and/or spheroidal at 17.5 degrees C. While phagocytosis at temperatures of 8 and 17.5 degrees C amounted to 85.8 and 83.1% of the activity observed at 37 degrees C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of 20 degrees C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2023 Electronic address https://www.frontiersin.org/articles/10.3389/fvets.2022.978756/full
Number of the records: 1