Number of the records: 1
Antimicrobial peptide in polymethylmethacrylate bone cement as a prophylaxis of infectious complications in orthopedics–an experiment in a murine model
- 1.
SYSNO ASEP 0557909 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antimicrobial peptide in polymethylmethacrylate bone cement as a prophylaxis of infectious complications in orthopedics–an experiment in a murine model Author(s) Melicherčík, P. (CZ)
Kotaška, K. (CZ)
Jahoda, D. (CZ)
Landor, I. (CZ)
Čeřovský, Václav (UOCHB-X) RID, ORCIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 67, č. 5 (2022), s. 785-791Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords antimicrobial peptide ; polymethylmethacrylate bone cement ; PMMA implants ; implant-related infections ; bacterial biofilm ; murine model OECD category Microbiology R&D Projects NV16-27726A GA MZd - Ministry of Health (MZ) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000802309600001 EID SCOPUS 85130760012 DOI 10.1007/s12223-022-00979-0 Annotation Polymethylmethacrylate (PMMA) bone cement mixed with antibiotics is used in orthopedic surgery to cope with implant-related infections which are typically associated with the formation of bacterial biofilms. Taking into account the growing bacterial resistance to current antibiotics, we examined here the efficacy of a selected antimicrobial peptide (AMP) mixed into the bone cement to inhibit bacterial adhesion and the consequent biofilm formation on its surface. In particular, we followed the formation of bacterial biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on implants made from PMMA bone cement loaded with AMP composed of 12 amino acid residues. This was evaluated by CFU counting of bacteria released by sonication from the biofilms formed on their surfaces after these implants were retrieved from the infected murine femoral canals. The AMP loaded in these model implants prevented adhesion of MRSA and the subsequent formation of MRSA biofilm on the surfaces of more than 80% of these implants, whereas biofilms did form on control implants made from the plain cement. The results of our experiments performed in the murine femoral canal indicate the potential for this murine osteomyelitis model to mimic actual operations in orthopedics. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2023 Electronic address https://doi.org/10.1007/s12223-022-00979-0
Number of the records: 1