Number of the records: 1
Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model
- 1.
SYSNO ASEP 0556529 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model Author(s) Chlupáč, Jaroslav (FGU-C) ORCID, RID
Matějka, R. (CZ)
Koňařík, M. (CZ)
Novotný, R. (CZ)
Šimůnková, Z. (CZ)
Mrázová, I. (CZ)
Fabian, O. (CZ)
Zapletal, M. (CZ)
Pulda, Z. (CZ)
Lipenský, J. F. (CZ)
Štěpanovská, J. (CZ)
Hanzálek, K. (CZ)
Brož, Antonín (FGU-C) RID, ORCID, SAI
Novák, T. (CZ)
Lodererová, A. (CZ)
Voska, L. (CZ)
Adla, T. (CZ)
Froněk, J. (CZ)
Rozkot, M. (CZ)
Forostyak, S. (CZ)
Kneppo, P. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Pirk, J. (CZ)Number of authors 23 Article number 3310 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 6 (2022)Number of pages 29 s. Language eng - English Country CH - Switzerland Keywords blood vessel prosthesis ; decellularization ; cell seeding ; tissue engineering ; allograft ; heterograft ; pericardium ; adipose tissue-derived stromal cells ; Wharton's jelly mesenchymal stem cells OECD category Cardiac and Cardiovascular systems R&D Projects NV18-02-00422 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000775384600001 EID SCOPUS 85126683388 DOI 10.3390/ijms23063310 Annotation Background: Cardiovascular surgery is confronted by a lack of suitable materials for patch repair. Acellular animal tissues serve as an abundant source of promising biomaterials. The aim of our study was to explore the bio-integration of decellularized or recellularized pericardial matrices in vivo. Methods: Porcine (allograft) and ovine (heterograft, xenograft) pericardia were decellularized using 1% sodium dodecyl sulfate ((1) Allo-decel and (2) Xeno-decel). We used two cell types for pressure-stimulated recellularization in a bioreactor: autologous adipose tissue-derived stromal cells (ASCs) isolated from subcutaneous fat of pigs ((3) Allo-ASC and (4) Xeno-ASC) and allogeneic Wharton's jelly mesenchymal stem cells (WJCs) ((5) Allo-WJC and (6) Xeno-WJC). These six experimental patches were implanted in porcine carotid arteries for one month. For comparison, we also implanted six types of control patches, namely, arterial or venous autografts, expanded polytetrafluoroethylene (ePTFE Propaten(R) Gore(R)), polyethylene terephthalate (PET Vascutek(R)), chemically stabilized bovine pericardium (XenoSure(R)), and detoxified porcine pericardium (BioIntegral(R) NoReact(R)). The grafts were evaluated through the use of flowmetry, angiography, and histological examination. Results: All grafts were well-integrated and patent with no signs of thrombosis, stenosis, or aneurysm. A histological analysis revealed that the arterial autograft resembled a native artery. All other control and experimental patches developed neo-adventitial inflammation (NAI) and neo-intimal hyperplasia (NIH), and the endothelial lining was present. NAI and NIH were most prominent on XenoSure(R) and Xeno-decel and least prominent on NoReact(R). In xenografts, the degree of NIH developed in the following order: Xeno-decel > Xeno-ASC > Xeno-WJC. NAI and patch resorption increased in Allo-ASC and Xeno-ASC and decreased in Allo-WJC and Xeno-WJC. Conclusions: In our setting, pre-implant seeding with ASC or WJC had a modest impact on vascular patch remodeling. However, ASC increased the neo-adventitial inflammatory reaction and patch resorption, suggesting accelerated remodeling. WJC mitigated this response, as well as neo-intimal hyperplasia on xenografts, suggesting immunomodulatory properties. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://www.mdpi.com/1422-0067/23/6/3310
Number of the records: 1