Number of the records: 1
How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription
- 1.
SYSNO ASEP 0554698 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription Author(s) Hoboth, Peter (UMG-J) ORCID
Sebesta, O. (CZ)
Hozák, Pavel (UMG-J) RID, ORCIDNumber of authors 3 Article number 6694 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 13 (2021)Number of pages 19 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords cell nucleus ; gene expression ; transcription foci ; transcription factors ; super-resolution microscopy ; structured illumination ; stimulated emission depletion ; stochastic optical reconstruction ; photoactivation Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1220 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-05608S GA ČR - Czech Science Foundation (CSF) GA18-19714S GA ČR - Czech Science Foundation (CSF) LTC19048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000671174300001 DOI 10.3390/ijms22136694 Annotation Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration of the spatial and temporal organization of gene expression. Although the spatial organization and dynamics of RNA polymerase II (RNAPII) transcription machinery have fundamental functional consequences for gene expression, its detailed studies have been abrogated by the limits of classical light microscopy for a long time. The advent of super-resolution microscopy (SRM) techniques allowed for the visualization of the RNAPII transcription machinery with nanometer resolution and millisecond precision. In this review, we summarize the recent methodological advances in SRM, focus on its application for studies of the nanoscale organization in space and time of RNAPII transcription, and discuss its consequences for the mechanistic understanding of gene expression. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/13/6694
Number of the records: 1