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Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections
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SYSNO ASEP 0553555 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections Author(s) Rebrošová, K. (CZ)
Bernatová, Silvie (UPT-D) RID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Uhlířová, M. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Holá, V. (CZ)
Růžička, F. (CZ)
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 9 Article number 339292 Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1191, 25 January (2022)Number of pages 10 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Raman spectroscopy ; Urinary tract infections ; Raman tweezers ; Optical trapping ; Bacteria Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects NU21-05-00341 GA MZd - Ministry of Health (MZ) EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 UT WOS 000744469700002 EID SCOPUS 85120916888 DOI 10.1016/j.aca.2021.339292 Annotation Urinary tract infections belong to the most common infections in the world. Besides communityacquired infections, nosocomial infections pose a high risk especially for patients having indwelling catheters, undergoing urological surgeries or staying at hospital for prolonged time. They can be often complicated by antimicrobial resistance and/or biofilm formation. Therefore, a rapid diagnostic tool enabling timely identification of a causative agent and its susceptibility to antimicrobials is a need. Raman spectroscopy appears to be a suitable method that allows rapid differentiation among microbes and provides a space for further analyses, such as determination of capability of biofilm formation or antimicrobial susceptibility/resistance in tested strains. Our work here presents a possibility to differ among most common microbes causing urinary tract infections (belonging to 20 species). We tested 254 strains directly from colonies grown on Mueller-Hinton agar plates. The results show that it is possible to distinguish among the tested species using Raman spectroscopy, which proves its great potential for future use in clinical diagnostics. Moreover, we present here a pilot study of a real-time analysis and identification (in less than 10 min) of single microbial cells directly in urine employing optical tweezers combined with Raman spectroscopy. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0003267021011181
Number of the records: 1