Quantifying Cyanothece growth under DIC limitation

Inomura, K.; Masuda, Takako; Eichner, Meri; Rabouille, S.; Zavřel, Tomáš; Červený, Jan; Vancová, Marie; Bernát, Gábor; Armin, G.; Claquin, P.; Kotabová, Eva; Stephan, S.; Suggett, D.J.; Deutsch, C.; Prášil, Ondřej

doi:https://dx.doi.org/10.1016/j.csbj.2021.11.036

Number of the records: 1

Quantifying Cyanothece growth under DIC limitation

1.

SYSNO ASEP	0550181
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quantifying Cyanothece growth under DIC limitation
Author(s)	Inomura, K. (US) Masuda, Takako (MBU-M)_ORCID Eichner, Meri (MBU-M)_{ORCID, RID} Rabouille, S. (FR) Zavřel, Tomáš (UEK-B)_{RID, SAI, ORCID} Červený, Jan (UEK-B)_{RID, ORCID, SAI} Vancová, Marie (BC-A)_{RID, ORCID} Bernát, Gábor (MBU-M)_ORCID Armin, G. (US) Claquin, P. (FR) Kotabová, Eva (MBU-M)_{RID, ORCID} Stephan, S. (DE) Suggett, D.J. (AU) Deutsch, C. (US) Prášil, Ondřej (MBU-M)_{RID, ORCID}
Source Title	Computational and Structural Biotechnology Journal. - : Elsevier - ISSN 2001-0370 Roč. 19, č. 2021 (2021), s. 6456-6464
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Cyanothece ; DIC ; CO2 ; Nitrogen fixation ; Nitrate ; Diurnal Cyanothece ; Carbon ; Photosynthesis ; Quantitative model
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
Subject RIV - cooperation	Biology Centre (since 2006) - Cell Biology Global Change Research Institute - Microbiology, Virology
R&D Projects	GA20-17627S GA ČR - Czech Science Foundation (CSF)
	GA18-24397S GA ČR - Czech Science Foundation (CSF)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971 ; UEK-B - RVO:86652079 ; BC-A - RVO:60077344
UT WOS	000731411300006
EID SCOPUS	85120717280
DOI	10.1016/j.csbj.2021.11.036
Annotation	The photoautotrophic, unicellular N-2-fixer, Cyanothece, is a model organism that has been widely used to study photosynthesis regulation, the structure of photosystems, and the temporal segregation of carbon (C) and nitrogen (N) fixation in light and dark phases of the diel cycle. Here, we present a simple quantitative model and experimental data that together, suggest external dissolved inorganic carbon (DIC) concentration as a major limiting factor for Cyanothece growth, due to its high C-storage requirement. Using experimental data from a parallel laboratory study as a basis, we show that after the onset of the light period, DIC was rapidly consumed by photosynthesis, leading to a sharp drop in the rate of photosynthesis and C accumulation. In N-2-fixing cultures, high rates of photosynthesis in the morning enabled rapid conversion of DIC to intracellular C storage, hastening DIC consumption to levels that limited further uptake. The N-2-fixing condition allows only a small fraction of fixed C for cellular growth since a large fraction was reserved in storage to fuel night-time N-2 fixation. Our model provides a framework for resolving DIC limitation in aquatic ecosystem simulations, where DIC as a growth-limiting factor has rarely been considered, and importantly emphasizes the effect of intracellular C allocation on growth rate that varies depending on the growth environment. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2022
Electronic address	https://www.sciencedirect.com/science/article/pii/S2001037021005018?via%3Dihub

Number of the records: 1