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Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets
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SYSNO ASEP 0550089 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Secretory IgAN-glycans contribute to the protection againstE. coliO55 infection of germ-free piglets Author(s) Raskova Kafkova, L. (CZ)
Brokešová, D. (CZ)
Křupka, M. (CZ)
Stehlíková, Zuzana (MBU-M)
Dvořák, Jiří (MBU-M) RID, ORCID
Coufal, Štěpán (MBU-M) ORCID, RID
Fajstová, Alena (MBU-M) ORCID
Šrůtková, Dagmar (MBU-M) ORCID, RID
Štěpánová, Kateřina (MBU-M) ORCID
Hermanová, Petra (MBU-M) ORCID
Štěpánková, Renata (MBU-M) RID
Überall, I. (CZ)
Škarda, J. (CZ)
Novák, Z. (US)
Vannucci, Luca (MBU-M) RID, ORCID
Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
Jirásková Zákostelská, Zuzana (MBU-M) ORCID
Šinkora, Marek (MBU-M) RID, ORCID
Městecký, Jiří (MBU-M) ORCID
Raška, M. (CZ)Source Title Mucosal Immunology. - : Springer - ISSN 1933-0219
Roč. 14, č. 2 (2021), s. 511-522Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords escherichia-coli ; immunoglobulin-a ; o-glycosylation ; small-intestine ; hinge-region ; n-glycan ; component ; antibodies ; binding ; pig Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA17-11275S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000572598900001 EID SCOPUS 85091687047 DOI https://doi.org/10.1038/s41385-020-00345-8 Annotation Mucosal surfaces are colonized by highly diverse commensal microbiota. Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans. In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo. Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removedN-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenicE. coliO55 challenge. SIgA, Fab, and Fc-SC were protective, whereas removal ofN-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival. In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination ofE. coliO55. These findings highlight the role of SIgA-associatedN-glycans in protection. Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.nature.com/articles/s41385-020-00345-8
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