Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions

0545930 - ÚVGZ 2022 RIV NL eng J - Journal Article
Karlický, Václav - Materová Kmecová, Z. - Kurasová, Irena - Nezval, J. - Štroch, Michal - Garab, G. - Špunda, Vladimír
Accumulation of geranylgeranylated chlorophylls in the pigment-protein complexes of Arabidopsis thaliana acclimated to green light: effects on the organization of light-harvesting complex II and photosystem II functions.
Photosynthesis Research. Roč. 149, č. 1-2 (2021), s. 233-252. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA MŠMT(CZ) EF16_019/0000797; GA MŠMT(CZ) LM2018123
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : chloroplast thylakoid membranes * energy-transfer pathways * circular-dichroism * tobacco plants * photosynthetic activity * thermal-stability * alpha-tocopherol * rice mutants * reductase * fluorescence * Arabidopsis thaliana * Chlorophylls * Green light * Thylakoid membrane * Structure and function of photosystem II * Thermal stability
OECD category: Biophysics
Impact factor: 3.429, year: 2021
Method of publishing: Open access
https://link.springer.com/article/10.1007%2Fs11120-021-00827-1

Light quality significantly influences plant metabolism, growth and development. Recently, we have demonstrated that leaves of barley and other plant species grown under monochromatic green light (500-590 nm) accumulated a large pool of chlorophyll a (Chl a) intermediates with incomplete hydrogenation of their phytyl chains. In this work, we studied accumulation of these geranylgeranylated Chls a and b in pigment-protein complexes (PPCs) of Arabidopsis plants acclimated to green light and their structural-functional consequences on the photosynthetic apparatus. We found that geranylgeranylated Chls are present in all major PPCs, although their presence was more pronounced in light-harvesting complex II (LHCII) and less prominent in supercomplexes of photosystem II (PSII). Accumulation of geranylgeranylated Chls hampered the formation of PSII and PSI super- and megacomplexes in the thylakoid membranes as well as their assembly into chiral macrodomains, it also lowered the temperature stability of the PPCs, especially that of LHCII trimers, which led to their monomerization and an anomaly in the photoprotective mechanism of non-photochemical quenching. Role of geranylgeranylated Chls in adverse effects on photosynthetic apparatus of plants acclimated to green light is discussed.
Permanent Link: http://hdl.handle.net/11104/0322534