Number of the records: 1  

Electrodeposition of silver amalgam particles on screen-printed silver electrodes in voltammetric detection of 4-nitrophenol, bovine serum albumin and artificial nucleosides dTPT3 and d5SICS

  1. 1.
    SYSNO ASEP0544550
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleElectrodeposition of silver amalgam particles on screen-printed silver electrodes in voltammetric detection of 4-nitrophenol, bovine serum albumin and artificial nucleosides dTPT3 and d5SICS
    Author(s) Havranová-Vidláková, Pavlína (BFU-R) ORCID
    Fojt, Lukáš (BFU-R) RID, ORCID
    Kejík, Lukáš (BFU-R)
    Šikola, T. (CZ)
    Fojta, Miroslav (BFU-R) RID, ORCID
    Daňhel, Aleš (BFU-R) RID, ORCID
    Number of authors6
    Article number129921
    Source TitleSensors and Actuators B - Chemical. - : Elsevier
    Roč. 340, aug 2021 (2021)
    Number of pages9 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordselectrochemical analysis ; semisynthetic organism ; 4-Nitrophenol ; Artificial nucleoside ; Catalytic hydrogen evolution
    Subject RIVCG - Electrochemistry
    OECD categoryElectrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    R&D ProjectsGJ17-23634Y GA ČR - Czech Science Foundation (CSF)
    EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707
    UT WOS000652627400004
    EID SCOPUS85104082616
    DOI10.1016/j.snb.2021.129921
    AnnotationElectrodeposition of silver amalgam particles (AgAPs) on various substrates represents a prospective approach to the development of a novel detection system applicable in the study of various electrochemically active substances, including nucleic acids and proteins. Herein, a double pulse chronoamperometric deposition of AgAPs on in-house fabricated screen-printed silver electrodes (SPAgE) has been optimized using the voltammetric signal of a model electrochemically reducible organic nitro-compound, 4-nitrophenol (4-NP). The surface morphology of SPAgE-AgAP was monitored by scanning electron microscopy with an energy dispersive X-ray spectrometer. A compact three-electrode sensor consisting of the working (substrate) SPAgE, counter graphite, and pseudoreference Ag|AgCl electrodes was designed for analysis of electrochemically reducible compounds in a 96-well plate with about 150-mu l sample volume per well. Herein optimized SPAgE-AgAP allowed detection of 4-NP down to 1 mu mol l- 1 using cyclic voltammetry. Advantageously, differential pulse voltammetry at SPAgE-AgAP allowed highly sensitive detection of artificial nucleosides dTPT3 and d5SICS (developed to expand genetic alphabet in semi-synthetic organisms) using their catalytic hydrogen evolution signals, with limits of detection of 0.4 pmol l- 1. Moreover, these artificial nucleosides were easily detectable in the excess of natural nucleosides down to molar ratio 1:12000. Using constant current chronopotentiometric stripping and the catalytic peak H, we further demonstrated detection of a protein, bovine serum albumin, at the SPAgE-AgAP. Thus, we present the SPAgE-AgAP as a potent tool applicable in simple, fast, and sensitive electrochemical detection of reducible or catalytically active species, with prospective applications in hot research areas including chemical and synthetic biology.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2022
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S0925400521004901?via%3Dihub
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.