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Electrodeposition of silver amalgam particles on screen-printed silver electrodes in voltammetric detection of 4-nitrophenol, bovine serum albumin and artificial nucleosides dTPT3 and d5SICS
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SYSNO ASEP 0544550 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrodeposition of silver amalgam particles on screen-printed silver electrodes in voltammetric detection of 4-nitrophenol, bovine serum albumin and artificial nucleosides dTPT3 and d5SICS Author(s) Havranová-Vidláková, Pavlína (BFU-R) ORCID
Fojt, Lukáš (BFU-R) RID, ORCID
Kejík, Lukáš (BFU-R)
Šikola, T. (CZ)
Fojta, Miroslav (BFU-R) RID, ORCID
Daňhel, Aleš (BFU-R) RID, ORCIDNumber of authors 6 Article number 129921 Source Title Sensors and Actuators B - Chemical. - : Elsevier
Roč. 340, aug 2021 (2021)Number of pages 9 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords electrochemical analysis ; semisynthetic organism ; 4-Nitrophenol ; Artificial nucleoside ; Catalytic hydrogen evolution Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GJ17-23634Y GA ČR - Czech Science Foundation (CSF) EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000652627400004 EID SCOPUS 85104082616 DOI 10.1016/j.snb.2021.129921 Annotation Electrodeposition of silver amalgam particles (AgAPs) on various substrates represents a prospective approach to the development of a novel detection system applicable in the study of various electrochemically active substances, including nucleic acids and proteins. Herein, a double pulse chronoamperometric deposition of AgAPs on in-house fabricated screen-printed silver electrodes (SPAgE) has been optimized using the voltammetric signal of a model electrochemically reducible organic nitro-compound, 4-nitrophenol (4-NP). The surface morphology of SPAgE-AgAP was monitored by scanning electron microscopy with an energy dispersive X-ray spectrometer. A compact three-electrode sensor consisting of the working (substrate) SPAgE, counter graphite, and pseudoreference Ag|AgCl electrodes was designed for analysis of electrochemically reducible compounds in a 96-well plate with about 150-mu l sample volume per well. Herein optimized SPAgE-AgAP allowed detection of 4-NP down to 1 mu mol l- 1 using cyclic voltammetry. Advantageously, differential pulse voltammetry at SPAgE-AgAP allowed highly sensitive detection of artificial nucleosides dTPT3 and d5SICS (developed to expand genetic alphabet in semi-synthetic organisms) using their catalytic hydrogen evolution signals, with limits of detection of 0.4 pmol l- 1. Moreover, these artificial nucleosides were easily detectable in the excess of natural nucleosides down to molar ratio 1:12000. Using constant current chronopotentiometric stripping and the catalytic peak H, we further demonstrated detection of a protein, bovine serum albumin, at the SPAgE-AgAP. Thus, we present the SPAgE-AgAP as a potent tool applicable in simple, fast, and sensitive electrochemical detection of reducible or catalytically active species, with prospective applications in hot research areas including chemical and synthetic biology. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0925400521004901?via%3Dihub
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