Arabidopsis bZIP18 and bZIP52 accumulate in nuclei following heat stress where they regulate the expression of a similar set of genes

Wiese, Anna J.; Steinbachová, Lenka; Timofejeva, Ljudmilla; Čermák, V.; Klodová, Božena; Ganji, R.S.; Limones-Mendez, Mariana; Bokvaj, Pavel; Hafidh, Said; Potěšil, D.; Honys, David

doi:https://dx.doi.org/10.3390/ijms22020530

Number of the records: 1

Arabidopsis bZIP18 and bZIP52 accumulate in nuclei following heat stress where they regulate the expression of a similar set of genes

1.

SYSNO ASEP	0544535
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Arabidopsis bZIP18 and bZIP52 accumulate in nuclei following heat stress where they regulate the expression of a similar set of genes
Author(s)	Wiese, Anna J. (UEB-Q) Steinbachová, Lenka (UEB-Q)_{ORCID, RID} Timofejeva, Ljudmilla (UEB-Q)_ORCID Čermák, V. (CZ) Klodová, Božena (UEB-Q)_ORCID Ganji, R.S. (CZ) Limones-Mendez, Mariana (UEB-Q)_ORCID Bokvaj, Pavel (UEB-Q) Hafidh, Said (UEB-Q)_{RID, ORCID} Potěšil, D. (CZ) Honys, David (UEB-Q)_{RID, ORCID}
Number of authors	11
Article number	530
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 2 (2021)
Number of pages	24 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Arabidopsis ; bZIP ; Heat stress ; Localization ; Transcriptomics
OECD category	Plant sciences, botany
R&D Projects	EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC18043 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC20028 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA17-23183S GA ČR - Czech Science Foundation (CSF)
	GA17-23203S GA ČR - Czech Science Foundation (CSF)
	LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
UT WOS	000611888100001
EID SCOPUS	85099135055
DOI	10.3390/ijms22020530
Annotation	Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14–3–3 proteins, and bZIP18 and bZIP52 were found to bind 14–3–3 ε, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14–3–3 ε interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2022
Electronic address	http://doi.org/10.3390/ijms22020530

Number of the records: 1