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Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5
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SYSNO ASEP 0542935 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5 Author(s) Kopejtka, Karel (MBU-M) ORCID, RID
Zeng, Yonghui (MBU-M) ORCID
Kaftan, David (MBU-M) ORCID
Selyanin, Vadim (MBU-M) RID
Gardian, Zdenko (BC-A) RID
Tomasch, J. (DE)
Sommaruga, R. (AT)
Koblížek, Michal (MBU-M) RID, ORCIDArticle number 768 Source Title Microorganisms. - : MDPI
Roč. 9, č. 4 (2021)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords aerobic anoxygenic phototrophic bacteria ; bacteriochlorophyll a ; photosynthesis genes ; rhodopsin ; Sphingomonadaceae Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Biology Centre (since 2006) - Microbiology, Virology R&D Projects GJ18-14095Y GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000643305000001 EID SCOPUS 85103602575 DOI 10.3390/microorganisms9040768 Annotation An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenkollesee located in the Tyrolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). Its genomic DNA G + C content is 65.9%. Further, in silico DNA-DNA hybridization and calculation of the average nucleotide identity speaks for the close phylogenetic relationship of AAP5 and Sphingomonas glacialis. The high percentage (76.2%) of shared orthologous gene clusters between strain AAP5 and Sphingomonas paucimobilis NCTC 11030(T), the type species of the genus, supports the classification of the two strains into the same genus. Strain AAP5 was found to contain C-18:1 omega 7c (64.6%) as a predominant fatty acid (>10%) and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, six unidentified glycolipids, one unidentified phospholipid, and two unidentified lipids. The main respiratory quinone was ubiquinone-10. Strain AAP5 is a facultative photoheterotroph containing type-2 photosynthetic reaction centers and, in addition, contains a xathorhodopsin gene. No CO2-fixation pathways were found. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2076-2607/9/4/768
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