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Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction
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SYSNO ASEP 0532424 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction Author(s) Hrdlička, Vojtěch (UFCH-W) ORCID, RID, SAI
Barek, J. (CZ)
Navrátil, Tomáš (UFCH-W) RID, ORCIDArticle number 121594 Source Title Talanta. - : Elsevier - ISSN 0039-9140
Roč. 221, JAN 2021 (2021)Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords Liquid phase microextraction ; Homovanillic acid ; Hollow fiber ; Differential pulse voltammetry Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA20-01589S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000579910600084 EID SCOPUS 85091253335 DOI 10.1016/j.talanta.2020.121594 Annotation Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5). Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0310920
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