High Mobility Group Box 1 and TLR4 Signaling Pathway in Gnotobiotic Piglets Colonized/Infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. Typhimurium.

Šplíchal, Igor; Donovan, S.M.; Jeništová, Věra; Šplíchalová, Iva; Salmonová, H.; Vlková, E.; Neužil Bunešová, V.; Šinkora, Marek; Killer, Jiří; Skřivanová, E.; Šplíchalová, Alla

doi:https://dx.doi.org/10.3390/ijms20246294

Number of the records: 1

High Mobility Group Box 1 and TLR4 Signaling Pathway in Gnotobiotic Piglets Colonized/Infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. Typhimurium.

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SYSNO ASEP	0519835
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	High Mobility Group Box 1 and TLR4 Signaling Pathway in Gnotobiotic Piglets Colonized/Infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. Typhimurium.
Author(s)	Šplíchal, Igor (MBU-M)_RID Donovan, S.M. (US) Jeništová, Věra (MBU-M) Šplíchalová, Iva (UMG-J) Salmonová, H. (CZ) Vlková, E. (CZ) Neužil Bunešová, V. (CZ) Šinkora, Marek (MBU-M)_{RID, ORCID} Killer, Jiří (UZFG-Y)_{RID, ORCID} Skřivanová, E. (CZ) Šplíchalová, Alla (MBU-M)_{RID, ORCID}
Article number	6294
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 20, Č. 24 (2019)
Number of pages	20 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Escherichia coli Nissle 1917 ; Lactobacillus amylovorus ; Lactobacillus mucosae ; Salmonella Typhimurium
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Institute of Molecular Genetics - Biochemistry Institute of Animal Physiology and Genetics - Microbiology, Virology
R&D Projects	GA13-08803S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 ; UZFG-Y - RVO:67985904
UT WOS	000506840100187
EID SCOPUS	85076856092
DOI	10.3390/ijms20246294
Annotation	High mobility group box 1 (HMGB1) is a DNA-binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll-like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4-related molecules (MD-2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT-qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST-infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal/probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST-infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2020
Electronic address	https://www.mdpi.com/1422-0067/20/24/6294

Number of the records: 1