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Serum proteomic analysis of melanoma patients with immunohistochemical profiling of primary melanomas and cultured cells: Pilot study
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SYSNO ASEP 0518779 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Serum proteomic analysis of melanoma patients with immunohistochemical profiling of primary melanomas and cultured cells: Pilot study Author(s) Kučera, J. (CZ)
Strnadová, K. (CZ)
Dvořánková, B. (CZ)
Lacina, L. (CZ)
Krajsová, I. (CZ)
Štork, J. (CZ)
Kovářová, Hana (UZFG-Y) RID, ORCID
Kupcová Skalníková, Helena (UZFG-Y) RID, ORCID
Vodička, Petr (UZFG-Y) ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Dundr, P. (CZ)
Smetana, K. Jr. (CZ)
Kodet, O. (CZ)Source Title Oncology Reports - ISSN 1021-335X
Roč. 42, č. 5 (2019), s. 1793-1804Number of pages 12 s. Publication form Print - P Language eng - English Country GR - Greece Keywords melanoma ; inflammation ; microenvironment Subject RIV FD - Oncology ; Hematology OECD category Oncology R&D Projects LO1609 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000785 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000490982300015 EID SCOPUS 85072621749 DOI 10.3892/or.2019.7319 Annotation The steadily increasing incidence of malignant melanoma (MM) and its aggressive behaviour makes this tumour an attractive cancer research topic. The tumour microenvironment is being increasingly recognised as a key factor in cancer biology, with an impact on proliferation, invasion, angiogenesis and metastatic spread, as well as acquired therapy resistance. Multiple bioactive molecules playing cooperative roles promote the chronic inflammatory milieu in tumours, making inflammation a hallmark of cancer. This specific inflammatory setting is evident in the affected tissue. However, certain mediators can leak into the systemic circulation and affect the whole organism. The present study analysed the complex inflammatory response in the sera of patients with MM of various stages. Multiplexed proteomic analysis (Luminex Corporation) of 31 serum proteins was employed. These targets were observed in immunohistochemical profiles of primary tumours from the same patients. Furthermore, these proteins were analysed in MM cell lines and the principal cell population of the melanoma microenvironment, cancer-associated fibroblasts. Growth factors such as hepatocyte growth factor, granulocyte-colony stimulating factor and vascular endothelial growth factor, chemokines RANTES and interleukin (IL)-8, and cytokines IL-6, interferon-alpha and IL-1 receptor antagonist significantly differed in these patients compared with the healthy controls. Taken together, the results presented here depict the inflammatory landscape that is altered in melanoma patients, and highlight potentially relevant targets for therapy improvement. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address https://www.spandidos-publications.com/10.3892/or.2019.7319
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