Number of the records: 1
The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo
- 1.
SYSNO ASEP 0518345 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo Author(s) Pourová, J. (CZ)
Applová, L. (CZ)
Macáková, K. (CZ)
Vopršalová, M. (CZ)
Migkos, T. (CZ)
Bentanachs, R. (CZ)
Biedermann, David (MBU-M) RID, ORCID
Petrásková, Lucie (MBU-M) ORCID
Tvrdý, V. (CZ)
Hrubša, M. (CZ)
Karlíčková, J. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Valentová, Kateřina (MBU-M) RID, ORCID
Mladěnka, P. (CZ)Article number 2286 Source Title Nutrients. - : MDPI
Roč. 11, č. 10 (2019)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords Silymarin ; Milk Thiste ; Flavonolignans Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems R&D Projects GA18-00121S GA ČR - Czech Science Foundation (CSF) LTC18003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC18071 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000498227300027 EID SCOPUS 85072847662 DOI 10.3390/nu11102286 Annotation Silymarin is a traditional drug and food supplement employed for numerous liver disorders. The available studies indicate that its activities may be broader, in particular due to claimed benefits in some cardiovascular diseases, but the contributions of individual silymarin components are unclear. Therefore, we tested silymarin flavonolignans as pure diastereomers as well as their sulfated metabolites for potential vasorelaxant and antiplatelet effects in isolated rat aorta and in human blood, respectively. Eleven compounds from a panel of 17 tested exhibited a vasorelaxant effect, with half maximal effective concentrations (EC50) ranging from 20 to 100 µM, and some substances retained certain activity even in the range of hundreds of nM. Stereomers A were generally more potent as vasorelaxants than stereomers B. Interestingly, the most active compound was a metabolite-silychristin-19-O-sulfate. Although initial experiments showed that silybin, 2,3-dehydrosilybin, and 2,3-dehydrosilychristin were able to substantially block platelet aggregation, their effects were rapidly abolished with decreasing concentration, and were negligible at concentrations ≤100 µM. In conclusion, metabolites of silymarin flavonolignans seem to have biologically relevant vasodilatory properties, but the effect of silymarin components on platelets is low or negligible. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://www.mdpi.com/2072-6643/11/10/2286
Number of the records: 1