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Purification of lutein from the green microalgae Chlorella vulgaris by integrated use of a new extraction protocol and a multi-injection high performance counter-current chromatography (HPCCC)
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SYSNO ASEP 0507964 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Purification of lutein from the green microalgae Chlorella vulgaris by integrated use of a new extraction protocol and a multi-injection high performance counter-current chromatography (HPCCC) Author(s) Fábryová, Tereza (MBU-M) ORCID
Cheel, José (MBU-M) RID, ORCID
Kubáč, David (MBU-M) RID, ORCID
Hrouzek, Pavel (MBU-M) ORCID
Vu, Dai Long (MBU-M) ORCID
Tůmová, Lenka (MBU-M)Article number 101574 Source Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 41, AUG 2019 (2019)Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords Lutein ; Microalgae ; Chlorella vulgaris Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) FV10155 GA MPO - Ministry of Industry and Trade (MPO) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000472593800041 EID SCOPUS 85067057566 DOI 10.1016/j.algal.2019.101574 Annotation Lutein is a natural carotenoid with health promoting properties whose commercial source is the yellow Marigold flower, but its production is limited by climate, planting area and high labor costs. The microalga Chlorella vulgaris is a promising lutein source however, no commercial lutein production uses microalgae. In the present study, the lower phase (LP4) of the biphasic solvent system composed of n-heptane-ethanol-water (5:4:1.5, v/v/v) was used both as a solvent for microalgae biomass extraction and as a mobile phase for lutein isolation using high performance counter-current chromatography (HPCCC). The ultrasound-assisted extraction of biomass with LP4 for 30 min led to an extract with a lutein content (3.20 mg/g dried biomass) that was higher than those obtained by the reference solvents tetrahydrofuran and dichloromethane. A multi-injection HPCCC method was developed for lutein isolation, where LP4 was pumped as the mobile phase at a flow rate of 8 mL/min, whereas its upper phase was the stationary phase. In total, 2 g of C. vulgaris extract were processed affording 60 mg of lutein (92% purity), which was further cleaned up by gel permeation chromatography yielding 50 mg of lutein (97% purity). The chemical identity of the purified compound was confirmed by mass spectrometry and absorption spectroscopy in comparison with an authentic standard. The developed HPCCC method may serve as a reference for promoting the development of a new production model of microalgae-based lutein. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S2211926419303418?via%3Dihub
Number of the records: 1