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Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields
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SYSNO ASEP 0507886 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields Author(s) Chafai, Djamel Eddine (URE-Y)
Sulimenko, Vadym (UMG-J) RID, ORCID
Havelka, Daniel (URE-Y) RID
Kubínová, Lucie (FGU-C) RID, ORCID
Dráber, Pavel (UMG-J) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 6 Article number e1903636 Source Title Advanced Materials. - : Wiley - ISSN 0935-9648
Roč. 31, č. 39 (2019)Number of pages 7 s. Publication form Print - P Language eng - English Country DE - Germany Keywords nanosecond pulsed electric field ; microtubules ; self-assembly ; tubulin Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering Subject RIV - cooperation Institute of Molecular Genetics - Cell Biology
Institute of Physiology - Cell BiologyR&D Projects GA17-11898S GA ČR - Czech Science Foundation (CSF) GA18-27197S GA ČR - Czech Science Foundation (CSF) GA18-23597S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support URE-Y - RVO:67985882 ; UMG-J - RVO:68378050 ; FGU-C - RVO:67985823 UT WOS 000481222200001 EID SCOPUS 85070672049 DOI 10.1002/adma.201903636 Annotation Tubulin self-assembly into microtubules is a fascinating natural phenomenon. Its importance is not just crucial for functional and structural biological processes, but it also serves as an inspiration for synthetic nanomaterial innovations. The modulation of the tubulin self-assembly process without introducing additional chemical inhibitors/promoters or stabilizers has remained an elusive process. This work reports a versatile and vigorous strategy for controlling tubulin self-assembly by nanosecond electropulses (nsEPs). The polymerization assessed by turbidimetry is dependent on nsEPs dosage. The kinetics of microtubules formation is tightly linked to the nsEPs effects on structural properties of tubulin, and tubulin-solvent interface, assessed by autofluorescence, and the zeta potential. Moreover, the overall size of tubulin assessed by dynamic light scattering is affected as well. Additionally, atomic force microscopy imaging reveals the formation of different assemblies reflecting applied nsEPs. It is suggested that changes in C-terminal modification states alter tubulin polymerization-competent conformations. Although the assembled tubulin preserve their integral structure, they might exhibit a broad range of new properties important for their functions. Thus, these transient conformation changes of tubulin and their collective properties can result in new applications Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201903636
Number of the records: 1