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Intrinsic Electrocatalysis in DNA
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SYSNO ASEP 0506881 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Intrinsic Electrocatalysis in DNA Author(s) Paleček, Emil (BFU-R) RID, ORCID
Bartošík, M. (CZ)Number of authors 2 Source Title ChemElectroChem. - : Wiley - ISSN 2196-0216
Roč. 5, č. 6 (2018), s. 936-942Number of pages 7 s. Publication form Print - P Language eng - English Country DE - Germany Keywords polarographic-behavior ; stripping voltammetry ; hybridization Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GA17-08971S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000428374400014 DOI 10.1002/celc.201700888 Annotation We show, for the first time, that DNA produces an electrocatalytic square wave voltammetric reduction peak or chronopotentiometric peak H-DNA at mercury and solid amalgam electrodes. These peaks are attributed to the catalytic hydrogen evolution reaction, allowing label-free detection of chromosomal DNA and oligodeoxynucleotides (ODNs) below parts per million and nanomolar levels, respectively. By using peak H-DNA at high current densities, native chromosomal DNA was distinguished from its denatured form. With this method, damage of DNA from human cancer cells by ionizing radiation or sonication was detected. Sensitivity of the DNA determination was greatly enhanced in the presence of [Co(NH3)(6)](3+). Chromosomal DNA and ODNs can be detected at concentrations 2-3 orders lower than any earlier voltammetric or chronopotentiometric label-free methods. In the presence of 4 mM [Co(NH3)(6)](3+), DNA from human cancer cells was easily detected at 5ng/mL, that is, below the concentration level of cell-free DNA in body fluids. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/epdf/10.1002/celc.201700888
Number of the records: 1