Number of the records: 1
Genome-wide C-SWAT library for high-throughput yeast genome tagging
- 1.
SYSNO ASEP 0506546 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genome-wide C-SWAT library for high-throughput yeast genome tagging Author(s) Meurer, M. (DE)
Duan, Y. (DE)
Sass, E. (IL)
Kats, I. (DE)
Herbst, K. (DE)
Buchmuller, B.C. (DE)
Dederer, V. (DE)
Huber, F. (DE)
Kirrmaier, D. (DE)
Štefl, Martin (UFCH-W)
Van Laer, K. (DE)
Dick, T.P. (DE)
Lemberg, M. K. (DE)
Khmelinskii, A. (DE)
Levy, E.D. (IL)
Knop, M. (DE)Source Title Nature Methods. - : Nature Publishing Group - ISSN 1548-7091
Roč. 15, č. 8 (2018), s. 598-600Number of pages 3 s. Language eng - English Country GB - United Kingdom Keywords green fluorescent protein ; saccharomyces-cerevisiae ; gene deletion ; global analysis ; budding yeast ; pcr ; expression Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000440334000019 EID SCOPUS 85049640566 DOI 10.1038/s41592-018-0045-8 Annotation Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2020 Electronic address http://hdl.handle.net/11104/0297778
Number of the records: 1