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Dose-Rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation
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SYSNO ASEP 0496971 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dose-Rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation Author(s) Vyšín, Luděk (FZU-D) RID, ORCID
Burian, Tomáš (FZU-D) RID, ORCID
Ukraintsev, Egor (FZU-D) RID, ORCID
Davídková, Marie (UJF-V) RID, ORCID, SAI
Grisham, M. E. (US)
Heinbuch, S. (US)
Rocca, J.J. (US)
Juha, Libor (FZU-D) RID, ORCID, SAINumber of authors 8 Source Title Radiation Research. - : Radiation Research Society - ISSN 0033-7587
Roč. 189, č. 5 (2018), s. 466-476Number of pages 11 s. Language eng - English Country US - United States Keywords breaking DNA strands ; short pulses ; extreme ultraviolet radiation Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) Subject RIV - cooperation Nuclear Physics Institute - Nuclear, Atomic and Molecular Physics, Colliders R&D Projects GA13-28721S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FZU-D - RVO:68378271 ; UJF-V - RVO:61389005 UT WOS 000430572800003 EID SCOPUS 85045934898 DOI 10.1667/RR14825.1 Annotation Dose-rate effects on strand break formation in plasmid DNA induced by pulsed extreme ultraviolet (XUV) radiation were examined. Dose delivered to the target molecule was controlled by attenuating the incident photon flux using aluminum filters as well as by changing the DNA/buffer-salt ratio in the irradiated sample. Irradiated samples were examined using agarose gel electrophoresis. Yields of single- and double-strand breaks (SSBs and DSBs) were determined as a function of the incident photon fluence. Damaged DNA was inspected by means of atomic force microscopy (AFM). Both SSB and DSB yields decreased with dose rate increase. Quantum yields of SSBs at the highest photon fluence were comparable to yields of DSBs found after synchrotron irradiation. In conclusion, complex and/or clustered damages other than cross-links do not appear to be induced under the radiation conditions applied in this study. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2019 Electronic address https://doi.org/10.1667/RR14825.1
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