Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints

Bezawork-Geleta, A.; Wen, H.; Dong, L. F.; Yan, B.; Vider, J.; Boukalová, Štěpána; Krobová, Linda; Váňová, Kateřina; Zobalová, Renata; Sobol, Margaryta; Hozák, Pavel; Novais, Silvia Magalhaes; Caisova, V.; Abaffy, Pavel; Naraine, Ravindra; Pang, Y.; Zaw, T.; Zhang, P.; Šindelka, Radek; Kubista, Mikael; Zuryn, S.; Molloy, M. P.; Berridge, M.V.; Pacak, K.; Rohlena, Jakub; Park, S.; Neužil, Jiří

doi:https://dx.doi.org/10.1038/s41467-018-04603-z

Number of the records: 1

Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints

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SYSNO ASEP	0492386
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints
Author(s)	Bezawork-Geleta, A. (AU) Wen, H. (CN) Dong, L. F. (AU) Yan, B. (AU) Vider, J. (AU) Boukalová, Štěpána (BTO-N) Krobová, Linda (BTO-N) Váňová, Kateřina (BTO-N) Zobalová, Renata (BTO-N)_RID Sobol, Margaryta (UMG-J)_RID Hozák, Pavel (UMG-J)_{RID, ORCID} Novais, Silvia Magalhaes (BTO-N) Caisova, V. (US) Abaffy, Pavel (BTO-N) Naraine, Ravindra (BTO-N) Pang, Y. (US) Zaw, T. (AU) Zhang, P. (AU) Šindelka, Radek (BTO-N)_RID Kubista, Mikael (BTO-N)_RID Zuryn, S. (AU) Molloy, M. P. (AU) Berridge, M.V. (NZ) Pacak, K. (US) Rohlena, Jakub (BTO-N)_{RID, ORCID} Park, S. (KR) Neužil, Jiří (BTO-N)_RID
Number of authors	27
Article number	2221
Source Title	Nature Communications. - : Nature Publishing Group Roč. 9, JUN 7 2018 (2018)
Number of pages	17 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	ELECTRON-TRANSPORT CHAIN ; PYRUVATE-KINASE M2 ; MITOCHONDRIAL-DNA MUTATIONS
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Institute of Molecular Genetics
R&D Projects	GA15-02203S GA ČR - Czech Science Foundation (CSF)
	NV17-30138A GA MZd - Ministry of Health (MZ)
	GA17-20904S GA ČR - Czech Science Foundation (CSF)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA16-22823S GA ČR - Czech Science Foundation (CSF)
Institutional support	BTO-N - RVO:86652036 ; UMG-J - RVO:68378050
UT WOS	000434379800018
EID SCOPUS	85048232435
DOI	10.1038/s41467-018-04603-z
Annotation	Cell growth and survival depend on a delicate balance between energy production and synthesis of metabolites. Here, we provide evidence that an alternative mitochondrial complex II (CII) assembly, designated as CIIlow, serves as a checkpoint for metabolite biosynthesis under bioenergetic stress, with cells suppressing their energy utilization by modulating DNA synthesis and cell cycle progression. Depletion of CIIlow leads to an imbalance in energy utilization and metabolite synthesis, as evidenced by recovery of the de novo pyrimidine pathway and unlocking cell cycle arrest from the S-phase. In vitro experiments are further corroborated by analysis of paraganglioma tissues from patients with sporadic, SDHA and SDHB mutations. These findings suggest that CIIlow is a core complex inside mitochondria that provides homeostatic control of cellular metabolism depending on the availability of energy.
Workplace	Institute of Biotechnology
Contact	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Year of Publishing	2019

Number of the records: 1