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Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae
- 1.0492148 - BÚ 2019 RIV AT eng J - Journal Article
Do, D. - Záveská Drábková, Lenka
Herbarium tale: the utility of dry specimens for DNA barcoding Juncaceae.
Plant Systematics and Evolution. Roč. 304, č. 2 (2018), s. 281-294. ISSN 0378-2697. E-ISSN 1615-6110
R&D Projects: GA ČR(CZ) GA16-14649S; GA ČR(CZ) GAP506/11/0774
Institutional support: RVO:67985939
Keywords : DNA barcoding * dry plants * herbarium specimens * Juncaceae
OECD category: Plant sciences, botany
Impact factor: 1.585, year: 2018
The herbarium specimens amplified well and may thus be successfully applied for both phylogenetic analyses and barcoding for the Juncaceae family. Amplifying DNA was more difficult from dried herbarium specimens than fresh samples but could be successful in most cases when appropriate internal primers were designed or methods were optimised. Using the set of universal primers recommended by the Consortium for the Barcode of Life and designing specific primers for a particular group of interest were both useful. Specimen age and amplicon length had limited detrimental effects on amplification success for most of the Juncaceae loci tested.
Permanent Link: http://hdl.handle.net/11104/0285733
Number of the records: 1