Mannan-based conjugates as a multimodal imaging platform for lymph nodes

0489337 - ÚMCH 2019 RIV GB eng J - Journal Article
Rabyk, Mariia - Galisová, A. - Jirátová, M. - Patsula, Vitalii - Srbová, Linda - Loukotová, Lenka - Parnica, Jozef - Jirák, D. - Štěpánek, Petr - Hrubý, Martin
Mannan-based conjugates as a multimodal imaging platform for lymph nodes.
Journal of Materials Chemistry B. Roč. 6, č. 17 (2018), s. 2584-2596. ISSN 2050-750X. E-ISSN 2050-7518
R&D Projects: GA MZd(CZ) NV15-25781A
Institutional support: RVO:61389013
Keywords : polysaccharide modification * mannan * multimodal imaging
OECD category: Pharmacology and pharmacy
Impact factor: 5.047, year: 2018

We show that mannan-based conjugates possess exceptional features for multimodal imaging because of their biocompatibility, biodegradability and self-targeting properties. Two new mannan conjugates, containing a gadolinium complex and a fluorescent probe, one based only on polysaccharide and the other one comprising polysaccharide with poly(2-methyl-2-oxazoline) grafts, were prepared and simultaneously visualized in vitro and in vivo by magnetic resonance and fluorescence imaging. The synthesis of these mannan-based complexes was based on alkylation with allyl bromide or grafting with poly(2-methyl-2-oxazoline) chains, followed by a thiol–ene click reaction with cysteamine to introduce primary amino groups into their structure. Finally, the obtained conjugates were functionalized with contrast labels using the corresponding N-hydroxysuccinimide esters. When used to detect lymph nodes, the polymers showed better imaging properties than a commercially available contrast agent.
Permanent Link: http://hdl.handle.net/11104/0283943