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Detection of G-protein coupled estrogen receptor (GPER) in the male reproductive tissue and spermatic cells during their development and maturation in pigs.\n\n
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SYSNO ASEP 0474844 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Detection of G-protein coupled estrogen receptor (GPER) in the male reproductive tissue and spermatic cells during their development and maturation in pigs.
Author(s) Krejčířová, R. (CZ)
Sommerová, V. (CZ)
Rajmon, R. (CZ)
Postlerová, Pavla (BTO-N) ORCID, RIDNumber of authors 4 Source Title Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR v. v. i., 2017 / Kubátová alena
S. 26-26Number of pages 1 s. Action XXIIIrd Symposium of Immunology and Biology of Reproduction Event date 18.05.2017 - 20.05.2017 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords estrogens ; estrogen receptors ; GPER ; spermatozoa ; sperm capacitation ; sperm maturation Subject RIV EC - Immunology OECD category Biochemical research methods R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation Effect of estrogenic substances on the male reproductive tissue and development of gametes is an essential for the reproductive success. Estrogens affect the target cells via estrogen receptors (ERs) by both genomic and non-genomic pathways. The G-protein coupled estrogen receptor (GPER) is a transmembrane receptor mediating rapid non-genomic responses of estrogens. ERs have been found in the male reproductive tract in many mammalian species including humans. GPER has not been detected in reproductive tissues of pigs yet. Its presence has been only described in boar ejaculated spermatozoa (Rago et al., J Anat 2014;224(6):732-6). We detected GPER in testicular and epididymal tissues and in epididymal spermatozoa using specific antibody G-19 by immunofluorescent microscopy. In the testis GPER was found in Leydig and spermatic cells. In epididymis, we immunolocalized GPER in plasma membrane of the secretory epithelium and in spermatozoa at the lumen of cauda epididymis tubule. In spermatozoa isolated from the epididymis GPER was localized in the acrosome and flagellum. Our results confirmed the presence GPER in boar epididymal sperm for the first time. The localization of GPER in the plasma membrane of epididymal secretory tissue and spermatozoa suggests the possible involvement of estrogens not only in the sperm development, but in the sperm post-testicular maturation.
Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2018
Number of the records: 1