Tools for biotechnological studies of the freshwater alga Nannochloropsis limnetica: antibiotic resistance and protoplast production

Noda, Judith; Mühlroth, A.; Bučinská, Lenka; Dean, Jason Lawrence; Bones, A.M.; Sobotka, Roman

doi:https://dx.doi.org/10.1007/s10811-016-1001-6

Number of the records: 1

Tools for biotechnological studies of the freshwater alga Nannochloropsis limnetica: antibiotic resistance and protoplast production

1.

SYSNO ASEP	0474411
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Tools for biotechnological studies of the freshwater alga Nannochloropsis limnetica: antibiotic resistance and protoplast production
Author(s)	Noda, Judith (MBU-M)_ORCID Mühlroth, A. (NO) Bučinská, Lenka (MBU-M)_RID Dean, Jason Lawrence (MBU-M) Bones, A.M. (NO) Sobotka, Roman (MBU-M)_{RID, ORCID}
Source Title	Journal of Applied Phycology. - : Springer - ISSN 0921-8971 Roč. 29, č. 2 (2017), s. 853-863
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Nannochloropsis ; Selection marker ; Antibiotics
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000399241500021
EID SCOPUS	84995459716
DOI	10.1007/s10811-016-1001-6
Annotation	Algae of the genus Nannochloropsis are attractive organisms for use in biotechnology due to their high lipid content. Genetic manipulation of marine Nannochloropsis species has already been reported, however, tools have not yet been developed to transform Nannochloropsis limnetica, the only known freshwater species of this genus. To establish N. limnetica as a model laboratory strain, we first tested the effects of 11 different antibiotics on growth of N. limnetica and the marine species N. oceanica and N. gaditana. These three microalgae responded very differently to antibiotic treatments, both in liquid cultures and on agar plates. In general, N. limnetica exhibited a much higher sensitivity to antibiotics than the marine strains, thus offering the potential for a large set of antibiotic resistance genes that may be applicable as artificial selection markers after transformation. We also developed a simple protocol using lysozyme to obtain high yields of viable N. limnetica protoplasts, as confirmed by flow cytometry and electron microscopy.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2018

Number of the records: 1