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Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles
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SYSNO ASEP 0471793 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Excretion/secretion products from Schistosoma mansoni adults, eggs and schistosomula have unique peptidase specificity profiles Author(s) Dvořák, Jan (UOCHB-X) ORCID
Fajtová, Pavla (UOCHB-X) RID, ORCID
Ulrychová, L. (CZ)
Leontovyč, Adrian (UOCHB-X) ORCID
Rojo-Arreola, L. (US)
Suzuki, B.M. (US)
Horn, Martin (UOCHB-X) RID, ORCID
Mareš, Michael (UOCHB-X) RID, ORCID
Craik, C. S. (US)
Caffrey, C. R. (US)
O'Donoghue, A.J. (US)Source Title Biochimie. - : Elsevier - ISSN 0300-9084
Roč. 122, Mar (2016), s. 99-109Number of pages 11 s. Language eng - English Country FR - France Keywords parasite ; fluke ; secretion ; excretion ; protease ; inhibitor Subject RIV CE - Biochemistry R&D Projects GAP302/11/1481 GA ČR - Czech Science Foundation (CSF) LO1302 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UOCHB-X - RVO:61388963 UT WOS 000370910700010 EID SCOPUS 84957794203 DOI 10.1016/j.biochi.2015.09.025 Annotation Schistosomiasis is one of a number of chronic helminth diseases of poverty that severely impact personal and societal well-being and productivity. Peptidases (proteases) are vital to successful parasitism, and Can modulate host physiology and immunology. Interference of peptidase action by specific drugs or vaccines can be therapeutically beneficial. To date, research on peptidases in the schistosome parasite has focused on either the functional characterization of individual peptidases or their annotation as part of global genome or transcriptome studies. We were interested in functionally characterizing the complexity of peptidase activity operating at the host parasite interface, therefore the excretory secretory products of key developmental stages of Schistosoma mansoni that parasitize the human were examined. Using class specific peptidase inhibitors in combination with a multiplex substrate profiling assay, a number of unique activities derived from endo- and exo-peptidases were revealed in the excretory-secretory products of schistosomula (larval migratory worms), adults and eggs. The data highlight the complexity of the functional degradome for each developmental stage of this parasite and facilitate further enquiry to establish peptidase identity, physiological and immunological function, and utility as drug or vaccine candidates. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2017
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