Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature

0469354 - ÚEM 2017 RIV GB eng J - Journal Article
Vymetálková, Veronika - Vodička, Pavel - Pardini, Barbara - Rosa, F. - Levý, M. - Schneiderová, M. - Liška, V. - Vodičková, Ludmila - Nilsson, T.K. - Farkas, S. A.
Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature.
Epigenomics. Roč. 8, č. 9 (2016), s. 1193-1207. ISSN 1750-1911. E-ISSN 1750-192X
R&D Projects: GA MZd(CZ) NT13424; GA MZd(CZ) NV15-27580A; GA ČR(CZ) GA15-08239S; GA MŠMT(CZ) LD14050
Institutional support: RVO:68378041
Keywords : DNA methylation * Illumina Human Methylation 450 BeadChip * rectal cancer
OECD category: Biochemistry and molecular biology
Impact factor: 4.541, year: 2016

Aim: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available. Materials & methods: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip. Results: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings. The BPIL3 and HBBP1 genes resulted hypomethylated in RC, whereas TIFPI2, ADHFE1, FLI1 and TLX1 were hypermethylated. An external validation by TCGA datasets confirmed the results. Conclusion: Our study, with external validation, has demonstrated the feasibility of using specific methylated DNA signatures for developing biomarkers in RC.
Permanent Link: http://hdl.handle.net/11104/0267155