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Polyhydroxyalkanoates in bacterial cells – more than just storage materials
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SYSNO ASEP 0465097 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Polyhydroxyalkanoates in bacterial cells – more than just storage materials Author(s) Obruča, S. (CZ)
Doskočil, L. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Sedláček, P. (CZ)
Mravec, F. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Kučera, J. (CZ)
Benešová, P. (CZ)
Márová, I. (CZ)Number of authors 10 Source Title Materials Science Forum, 851. - London : Trans Tech Publications, 2016 - ISSN 1662-9752 Pages s. 20-25 Number of pages 6 s. Publication form Print - P Action Conference on Chemistry and Life /6./ Event date 02.09.2016 - 04.09.2016 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CH - Switzerland Keywords polyhydroxyalkanoates ; stress survival ; bacterial cell cytoplasm ; analytical centrifugation ; Cryo-SEM Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-20645S GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 EID SCOPUS 84979084890 DOI 10.4028/www.scientific.net/MSF.851.20 Annotation Since polyhydroxyalkanoates (PHAs) belong among the most widespread storage materials within bacteria genus, it can be assumed that the ability of PHAs accumulation represent significant advantage in the natural environments. Of course, probably the most obvious is the possibility to utilize PHAs when external carbon sources are depleted. Nevertheless, it is likely that PHAs play much more complex role in the stress response of bacteria. Therefore, the aim of this work was to investigate possible influence of PHA accumulation in bacteria on physical properties of the cells and their cytoplasm with respect to possible stress survival. Cells of bacteria Cupriavidus necator H16 with various poly(3-hydroxybutyrate) (PHB) content (25 – 91% of cell dry weight) were subjected to analytical centrifugation employing LUMiSizer. We observed that slope of index instability of bacterial culture increased with rise of PHB content in cells. It indicates that accumulation of PHB granules in bacterial cells affects overall physico-mechanical properties of the cells in particular sedimentation potential and density which might influence their behavior in natural environment such as sedimentation, surface absorption and subsequent biofilm formation. Furthermore, when investigated by Cryo-SEM, the PHB containing cells showed needle-type plastic deformations while these structures were absent in the cells without polymer. This suggests that native intracellular PHB granules reveal completely different mechanical and physico-chemical properties than any other component of bacterial cytoplasm and their flexibility even in deeplyfrozen state is significantly higher than that of PHB isolated from bacterial cells. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2017
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