Architecture of the light-harvesting apparatus of the eustigmatophyte alga Nannochloropsis oceanica

0464890 - BC 2017 RIV NL eng J - Journal Article
Litvín, Radek - Bína, David - Herbstová, Miroslava - Gardian, Zdenko
Architecture of the light-harvesting apparatus of the eustigmatophyte alga Nannochloropsis oceanica.
Photosynthesis Research. Roč. 130, 1-3 (2016), s. 137-150. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA ČR(CZ) GP14-01377P
Institutional support: RVO:60077344
Keywords : Light harvesting * Thylakoid membrane * Vaucheriaxanthin * Violaxanthin–chlorophyll protein
Subject RIV: BO - Biophysics
Impact factor: 3.864, year: 2016

We present proteomic, spectroscopic, and phylogenetic analysis of light-harvesting protein (Lhc) function in oleaginous Nannochloropsis oceanica (Eustigmatophyta, Stramenopila). N. oceanica utilizes Lhcs of multiple classes: Lhcr-type proteins (related to red algae LHCI), Lhcv (VCP) proteins (violaxanthin-containing Lhcs related to Lhcf/FCP proteins of diatoms), Lhcx proteins (related to Lhcx/LhcSR of diatoms and green algae), and Lhc proteins related to Red-CLH of Chromera velia. Altogether, 17 Lhc-type proteins of the 21 known from genomic data were found in our proteomic analyses. Besides Lhcr-type antennas, a RedCAP protein and a member of the Lhcx protein subfamily were found in association with Photosystem I. The free antenna fraction is formed by trimers of a mixture of Lhcs of varied origins (Lhcv, Lhcr, Lhcx, and relatives of Red-CLH). Despite possessing several proteins of the Red-CLH-type Lhc clade, N. oceanica is not capable of chromatic adaptation under the same conditions as the diatom Phaeodactylum tricornutum or C. velia. In addition, a naming scheme of Nannochloropsis Lhcs is proposed to facilitate further work.
Permanent Link: http://hdl.handle.net/11104/0263638