Acquisition of exogenous haem is essential for tick reproduction

Perner, Jan; Sobotka, Roman; Šíma, Radek; Konvičková, Jitka; Sojka, Daniel; de Oliveira, P.L.; Hajdušek, Ondřej; Kopáček, Petr

doi:https://dx.doi.org/10.7554/eLife.12318

Number of the records: 1

Acquisition of exogenous haem is essential for tick reproduction

1.

SYSNO ASEP	0463424
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Acquisition of exogenous haem is essential for tick reproduction
Author(s)	Perner, Jan (BC-A)_{RID, ORCID} Sobotka, Roman (MBU-M)_{RID, ORCID} Šíma, Radek (BC-A)_{RID, ORCID} Konvičková, Jitka (BC-A) Sojka, Daniel (BC-A)_{RID, ORCID} de Oliveira, P.L. (BR) Hajdušek, Ondřej (BC-A)_{RID, ORCID} Kopáček, Petr (BC-A)_{RID, ORCID}
Source Title	eLife. - : eLife - ISSN 2050-084X Roč. 5, Mar 7 (2016), e12318
Number of pages	21 s.
Publication form	Online - E
Language	eng - English
Country	GB - United Kingdom
Keywords	biochemistry ; haem auxotrophy ; haem oxygenase ; haematophagy ; infectious disease ; iron metabolism ; microbiology ; ticks
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA13-11043S GA ČR - Czech Science Foundation (CSF)
	GP13-12816P GA ČR - Czech Science Foundation (CSF)
	GA14-33693S GA ČR - Czech Science Foundation (CSF)
	GP13-27630P GA ČR - Czech Science Foundation (CSF)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344 ; MBU-M - RVO:61388971
UT WOS	000373891500001
EID SCOPUS	84964301646
DOI	10.7554/eLife.12318
Annotation	Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. Unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding haem oxygenase. We demonstrated, by membrane feeding, that ticks do not acquire bioavailable iron from haemoglobin-derived haem. However, ticks require dietary haemoglobin as an exogenous source of haem since, feeding with haemoglobin-depleted serum led to aborted embryogenesis. Supplementation of serum with haemoglobin fully restored egg fertility. Surprisingly, haemoglobin could be completely substituted by serum proteins for the provision of amino-acids in vitellogenesis. Acquired haem is distributed by haemolymph carrier protein(s) and sequestered by vitellins in the developing oocytes. This work extends, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of haem and iron metabolism as rational targets for anti-tick interventions.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2017

Number of the records: 1