Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

Ujčíková, Hana; Vošahlíková, Miroslava; Roubalová, Lenka; Svoboda, Petr

doi:https://dx.doi.org/10.1016/j.jprot.2016.02.019

Number of the records: 1

Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

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SYSNO ASEP	0461865
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug
Author(s)	Ujčíková, Hana (FGU-C)_{RID, ORCID} Vošahlíková, Miroslava (FGU-C)_{RID, ORCID, SAI} Roubalová, Lenka (FGU-C)_{RID, ORCID, SAI} Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Journal of Proteomics. - : Elsevier - ISSN 1874-3919 Roč. 145, Aug 11 (2016), s. 11-23
Number of pages	13 s.
Language	eng - English
Country	NL - Netherlands
Keywords	morphine ; long-term exposure ; rat forebrain cortex ; post-nuclear supernatant ; MALDI-TOF MS/MS ; MaxLFQ
Subject RIV	CE - Biochemistry
R&D Projects	GBP304/12/G069 GA ČR - Czech Science Foundation (CSF)
	GAP207/12/0919 GA ČR - Czech Science Foundation (CSF)
Institutional support	FGU-C - RVO:67985823
UT WOS	000383931000003
EID SCOPUS	84959499628
DOI	10.1016/j.jprot.2016.02.019
Annotation	Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/−M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and (− M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with (− M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2017

Number of the records: 1