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Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo
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SYSNO ASEP 0451787 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo Author(s) Li, Y.J. (CN)
Guo, Y. (CN)
Yang, Q. (CN)
Weng, X. G. (CN)
Yang, L. (CN)
Wang, Y.J. (CN)
Chen, Y. (CN)
Zhang, D. (CN)
Li, Q. (CN)
Liu, X.C. (CN)
Kan, X.X. (CN)
Chen, X. (CN)
Zhu, X.X. (CN)
Kmoníčková, E. (CZ)
Zídek, Zdeněk (UEM-P)Number of authors 15 Source Title Toxicology and Applied Pharmacology. - : Elsevier - ISSN 0041-008X
Roč. 286, č. 3 (2015), s. 151-158Number of pages 8 s. Language eng - English Country US - United States Keywords Artemisia annua L. ; Flavonoids ; Casticin ; Chrysosplenol D ; Inflammation Subject RIV EC - Immunology Institutional support UEM-P - RVO:68378041 UT WOS 000356398300002 EID SCOPUS 84937972749 DOI 10.1016/j.taap.2015.04.005 Annotation Background: The aim of our experiments was to investigate the anti-inflammatory properties of casticin and chrysosplenol D, two flavonoids present in Artemisia annua L. /n/nMethods: Topical inflammation was induced in ICR mice using croton oil. Mice were then treated with casticin or chrysosplenol D. Cutaneous histological changes and edema were assessed. ICR mice were intragastrically administrated with casticin or chrysosplenol D followed by intraperitoneal injection of lipopolysaccharide (LPS). Mouse Raw264.7 macrophage cells were incubated with casticin or chrysosplenol D. Intracellular phosphorylation was detected, and migration was assessed by trans-well assay. HT-29/NF kappa B-luc cells were incubated with casticin or chrysosplenol D in the presence or absence of LPS, and NF-kappa B activation was quantified. /n/nResults: In mice, administration of casticin (0.5, 1 and 1.5 mu mol/cm(2)) and chrysosplenol D (1 and 1.5 mu mol/cm(2)) inhibited croton oil-induced ear edema (casticin: 29.39-64.95%; chrysosplenol D: 37.76-65.89%, all P < 0.05) in a manner similar to indomethacin (0.5, 1 and 1.5 mu mol/cm(2); 55.63-84.58%). Casticin (0.07, 0.13 and 027 mmol/kg) and chrysosplenol D (0.07, 0.14 and 0.28 mmol/kg) protected against LPS-induced systemic inflammatory response syndrome (SIRS) in mice (all P < 0.05), in a manner similar to dexamethasone (0.03 mmol/kg). Casticin and chrysosplenol D suppressed LPS-induced release of IL-1 beta, IL-6 and MCP-1, inhibited cell migration, and reduced LPS-induced I kappa B and c-JUN phosphorylation in Raw264.7 cells. JNK inhibitor SP600125 blocked the inhibitory effect of chrysosplenol Don cytokine release. /n Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2016
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