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Raman-tweezers in microfluidic system for analysis and sorting of living cells
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SYSNO ASEP 0451050 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Raman-tweezers in microfluidic system for analysis and sorting of living cells Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Ježek, Jan (UPT-D) RID, ORCID, SAI
Kaňka, Jan (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 4 Source Title Invitation and Programm 116th Annual Meeting of the DGaO. - Erlangen : DGaO, 2015
S. 24Number of pages 1 s. Publication form Print - P Action Annual Meeting of the DGaO /116./ Event date 26.05.2015-29.05.2015 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country DE - Germany Keywords optical systems ; spectroscopy ; laser technology Subject RIV BH - Optics, Masers, Lasers R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LD14069 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 Annotation We built an experimental apparatus for Raman analysis and sorting of optically trapped living cells (microorganisms, cell cultures) in microfluidic environment. Optical trapping allowed non-contact spatial confinement and separation of the analyzed cells, while Raman spectroscopy was used for determination of the chemical parameters of the cells. Special microfluidic chip serves to deliver the cells to the area of Raman-tweezers and to separate and isolate the selected cells. The system allows fully automatic analysis and sorting of living microorganisms according to selected parameters of the Raman spectrum. The selected cells can be easily removed from the microfluidic chip in a form of cell-containing agarose plug. This system may find its use in various biological, biotechnological and medical disciplines. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2016
Number of the records: 1