Direct covalent coupling of proteins to nanostructured plasma polymers: a route to tunable cell adhesion

0445012 - FGÚ 2016 RIV NL eng J - Journal Article
Melnichuk, I. - Choukourov, A. - Bilek, M. - Weiss, A. - Vandrovcová, Marta - Bačáková, Lucie - Hanuš, J. - Kousal, J. - Shelemin, A. - Solař, P. - Slavínská, D. - Biederman, H.
Direct covalent coupling of proteins to nanostructured plasma polymers: a route to tunable cell adhesion.
Applied Surface Science. Roč. 351, Oct 1 (2015), s. 537-545. ISSN 0169-4332. E-ISSN 1873-5584
R&D Projects: GA MZd(CZ) NT13297
Institutional support: RVO:67985823
Keywords : covalent binding * plasma polymers * MG-63 osteoblasts
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 3.150, year: 2015

Flat and nanostructured thin films were fabricated by deposition of ultrathin layer of hydrocarbon plasma polymer over polished silicon and over a pattern of 8 nm-thick poly(ethylene) islands on silicon. Linker-free radical-based covalent binding of bovine serum albumin and tropoelastin was confirmed for both types of films. The binding capability of albumin was found to be stable over many days of ambient air storage time. Tropoelastin-mediated flat plasma polymers favored adhesion and proliferation of osteoblast-like MG-63 cells. Nanostructured plasma polymers were multi-affine and their hierarchical surface represented an additional barrier for cell attachment
Permanent Link: http://hdl.handle.net/11104/0247432