Loss of loop adenines alters human telomere d[AG3(TTAG3)3] quadruplex folding

0440266 - BFÚ 2015 RIV GB eng J - Journal Article
Babinský, M. - Fiala, R. - Kejnovská, Iva - Bednářová, Klára - Marek, R. - Sagi, J. - Sklenář, V. - Vorlíčková, Michaela
Loss of loop adenines alters human telomere d[AG3(TTAG3)3] quadruplex folding.
Nucleic Acids Research. Roč. 42, č. 22 (2014), s. 14031-14041. ISSN 0305-1048. E-ISSN 1362-4962
R&D Projects: GA ČR(CZ) GAP205/12/0466
Institutional support: RVO:68081707
Keywords : human telomere * DNA quadruplex * cellular DNA
Subject RIV: BO - Biophysics
Impact factor: 9.112, year: 2014

corporated APs, one-by-one, in place of each of the three loop adenines of 5 -d[AG3(TTAG3)3] fragment of the human telomere sequence (htel-22). CDspectroscopy and electrophoretic results indicate that the AP site does not hamper the ability of the three modified sequences to fold into intramolecular quadruplexes in potassium solution. However, the equilibrium of quadruplex folds existing in the wild-type sequence is shifted toward higher population of parallel strands in the quadruplex structures at DNA strand concentrations close to 1 mM.
Permanent Link: http://hdl.handle.net/11104/0243385