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Raman tweezers in microfluidic systems for automatic analysis and sorting of living cells
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SYSNO ASEP 0436456 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman tweezers in microfluidic systems for automatic analysis and sorting of living cells Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID Number of authors 1 Source Title Sborník příspěvků multioborové konference Laser54. - Brno : Ústav přístrojové techniky AV ČR, 2014 - ISBN 978-80-87441-13-8 Pages s. 60 Number of pages 1 s. Publication form Print - P Action Laser54 Event date 29.10.2014-31.10.2014 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords laser trapping ; spectroscopy ; biophysics ; microfluidics Subject RIV BH - Optics, Masers, Lasers R&D Projects TA03010642 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LD14069 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 Annotation We have devised an automatic analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, together with computerized real time image analysis, spectra processing and micromanipulation. This device serves to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. The special microfluidic chip uses gravity to move the cells across the sorting area. Our system uses dedicated software to achieve fully automated spectral analysis and sorting. The devised system is a robust and universal platform for non-contact sorting of microobjects based on their chemical properties. It could find its use in many medical, biotechnological, and biological applications. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2015
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