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Modulation of the Early Inflammatory Microenvironment in the Alkali-Burned Eye by Systemically Administered Interferon-γ-Treated Mesenchymal Stromal Cells
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SYSNO ASEP 0432995 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modulation of the Early Inflammatory Microenvironment in the Alkali-Burned Eye by Systemically Administered Interferon-γ-Treated Mesenchymal Stromal Cells Author(s) Javorková, Eliška (UEM-P) RID
Trošan, Peter (UEM-P)
Zajícová, Alena (UEM-P) RID
Krulová, Magdaléna (UEM-P)
Hájková, M. (CZ)
Holáň, Vladimír (UEM-P) RIDNumber of authors 6 Source Title Stem Cells and Development. - : Mary Ann Liebert - ISSN 1547-3287
Roč. 23, č. 20 (2014), s. 2490-2500Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords mesenchymal stromal cells ; nitric oxide ; transforming growth factor beta Subject RIV EC - Immunology R&D Projects GAP304/11/0653 GA ČR - Czech Science Foundation (CSF) GAP301/11/1568 GA ČR - Czech Science Foundation (CSF) GA14-12580S GA ČR - Czech Science Foundation (CSF) NT14102 GA MZd - Ministry of Health (MZ) Institutional support UEM-P - RVO:68378041 UT WOS 000342614300008 EID SCOPUS 84913585238 DOI 10.1089/scd.2013.0568 Annotation The aim of this study was to investigate the effects of systemically administered bone-marrow-derived mesenchymal stromal cells (MSCs) on the early acute phase of inflammation in the alkali-burned eye. Mice with damaged eyes were either untreated or treated 24?h after the injury with an intravenous administration of fluorescent-dye-labeled MSCs that were unstimulated or pretreated with interleukin-1α (IL-1α), transforming growth factor-β (TGF-β), or interferon-γ (IFN-γ). Analysis of cell suspensions prepared from the eyes of treated mice on day 3 after the alkali burn revealed that MSCs specifically migrated to the damaged eye and that the number of labeled MSCs was more than 30-times higher in damaged eyes compared with control eyes. The study of the composition of the leukocyte populations within the damaged eyes showed that all types of tested MSCs slightly decreased the number of infiltrating lymphoid and myeloid cells, but only MSCs pretreated with IFN-γ significantly decreased the percentage of eye-infiltrating cells with a more profound effect on myeloid cells. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2015
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